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Methodsofscreening,culturing,improvementandstorageofmicroorganisms...
g)
after3daysoffermentation,determinetheethanol
contentinthesamplewiththeimmobilisedyeastand
compareitwiththecontrolsample;
h)
carryoutthenextfermentationcycle.Afterremoving
thefermentedwortandwashingthebeadsofthe
immobilizatewithsteriledistilledwater,introducefresh
mediuminthesamevolumeasinthefirstcycle.Repeat
thefermentationtestandethanolcontentanalysisafter
3daysoffermentation.Comparetheresultswiththe
firstandthecontrolsample.Thefollowingcyclescan
berepeatedseveraltimes,themechanicalstrength
ofthebeadsaswellastheenzymaticactivityofthe
immobilisedyeastisatleast12weeks.
2.
ImmobilisationofS.cerevisiaecellsinχ-carrageenan.
Preparetheyeastsuspensionasdescribedinpoint1a.
a)
Preparationoftheχ-carrageenansolution.Preparehot
(60°C)4%carrageenansolutionin0.9%NaCl;
b)
Thoroughlymixthewarmcarrageenansolution(40°C)
withtheyeastsuspensionataratioof9:1;maintainat
40°C;
c)
Placethecellsuspensioninthecarrageenanin
asyringe(asinpoint1d)anddropitinasterile2%
KClsolution.Leavethebeadsinthesolutionfor20
minutes.Rinsethebeadscreatedtwicewithsterile
distilledwater;
d)Continueasin1e-h.
1040Microorganismscultureinanaerobicconditons
INTRODUCTION
Anaerobicbacteriaplayakeyroleinthebiogeochemicalcycle
ofmanyimportantelements(e.g.carbon(C),nitrogen(N),
phosphorus(P),sulphur(S),iron(Fe)),contributingtothe
biologicalbalanceinbothnaturalandman-made(anthropogenic)
ecosystems.
Manyanaerobicspeciesarepathogensthatarethebasic
etiologicalagentofmanyhumanandanimalinfectiousdiseases.
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