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Methodsofscreening,culturing,improvementandstorageofmicroorganisms...
b)
streakingfromliquidmedium(10%inoculum)of
strainsisolatedfromthesoil-upto20mlofuniversal
mediumwithadditionofanthraceneorphenanthrene
ataconcentrationof0.5g/l.
Incubationfor96hat28°C(fungi)and30°C
(bacteria);
c)
extractionshouldbeperformedwithethylacetate.
Preparetheevaporatedextractforchromatographic
analyses(seeSection5.6.6);
d)
chromatographicanalysisforsamplesextractedwith
ethylacetateandconcentratedunderreducedpressure
isperformedusingagaschromatographcoupledto
amassspectrometerGC/MS(AgilentGC7890and
MS5975C)withHP5MScolumn(30m×0.25mm
×0.25mm)atatemperaturerangeof80-300°C(see
Section2.3)ataheliumflowof1ml/min.
Analysisofresults
Onthebasisoftheconductedanalyses,evaluatethesuitabilityof
theisolatedmicroorganismsfor:
1)productionofproteolyticenzymes;
2)productionofsubstancesofantagonisticactivity;
3)degradationofpetroleum-basedcompounds.
1030Methodsofculturingandstabilisaton
ofmicroorganismsunderaerobicconditons
(includingbioreactorsandimmobilisatoningels)
INTRODUCTION
Knowledgeofmicrobialphysiology,includinggrowthphysiology,
isessentialforpropercontroloftechnologicalprocessesin
whichmicroorganismsareused.Inbiotechnology,surfaceor
submergedculturingmethodscanbeusedforindustrialstrains.
Duetotheneedtohaverelativelylargeproductionfacilitiesand
usuallylowerprocessefficiency,surfacemethodsarerarely
usedtoday.Submergedculturesusebioreactors(fermenters)
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