Microbial biotechnology in the laboratory and practice

Jerzy Długoński

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ISBN/ISSN:

978-83-8220-420-9

DOI:

Wydawnictwo:

Wydawnictwo Uniwersytetu Łódzkiego

Rok wydania:

2021

Liczba stron:

552

XML:ISBN/ISSN:

ONIX MARC21

Bibliografia:

. Microbial biotechnology in the laboratory and practice. Red. Długoński, Jerzy . Łódź: Wydawnictwo Uniwersytetu Łódzkiego, 2021, 552 s. ISBN 978-83-8220-420-9

Bibliografia refWorks:

RT Book, Whole

SR Electronic(1)

A2 Długoński, J.

T1 Microbial biotechnology in the laboratory and practice

PB Wydawnictwo Uniwersytetu Łódzkiego

PP Łódź

YR 2021

SN 978-83-8220-420-9

Bibliografia BibTex:

@Book{ 253180, author = "", editor = "Długoński, Jerzy", title = "Microbial biotechnology in the laboratory and practice", publisher = "Wydawnictwo Uniwersytetu Łódzkiego", year = "2021", address = "Łódź", isbn = "978-83-8220-420-9" }

Bibliografia endNote:

TY - BOOK

AU -

ED - Długoński, Jerzy

TI - Microbial biotechnology in the laboratory and practice

PB - Wydawnictwo Uniwersytetu Łódzkiego

CY - Łódź

PY - 2021

SN - 978-83-8220-420-9

ER -

Netografia (standard APA):

. Red. Długoński, Jerzy. Microbial biotechnology in the laboratory and practice [baza danych online] Łódź: Wydawnictwo Uniwersytetu Łódzkiego, 2021 [dostęp: 19 08 2024]. Dostęp w Ibuk Libra: https://libra.ibuk.pl/reader/microbial-biotechnology-in-the-laboratory-and-practice-jerzy-dlugonski-253180.

revitalization, Biotechnology, microorganisms, biosynthesis, biodegradation, omics

In addition to the issues constituting the basis of microbial biotechnology, such as microorganisms culturing or their use in industry and environmental protection, the book includes modern analytical techniques known as „omics”, as we...

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ISBN/ISSN:

978-83-8220-420-9

DOI:

Wydawnictwo:

Wydawnictwo Uniwersytetu Łódzkiego

Rok wydania:

2021

Liczba stron:

552

XML:ISBN/ISSN:

ONIX MARC21

Foreword (J. Długoński) /11
Foreword to the handbook Microbiological Biotechnology. Exercises and specialist laboratories from 1997 (J. Długoński) /15
1. Methods of screening, culturing, improvement and storage of microorganisms of industrial importance /17
1.1. General characteristics of microorganisms used in biotechnological processes (J. Długoński) /19
1.2. Screening of microorganisms useful in microbiological processes (J. Długoński, K. Lisowska, N. Wrońska, K. Zawadzka, A. Felczak) /21
1.3. Methods of culturing and stabilisation of microorganisms under aerobic conditions (including bioreactors and immobilisation in gels) (J. Długoński, P. Bernat, K. Lisowska, S. Walisch) /33
1.4. Microorganism culture in anaerobic conditions (M. Krupiński) /47
1.5. Fungal protoplasts: release, properties and application (J. Długoński, K. Paraszkiewicz, M. Słaba, K Milczarek) /53
1.6. Strain development – mutagenesis, fusion and electroporation of protoplasts (J. Długoński,D. Wilmańska, S. Różalska, K. Lisowska, K. Milczarek) /58
1.7. Storage of industrial strains (K. Zawadzka, N. Wrońska, S. Walisch, K. Milczarek, D. Wilmańska) /74
Literature /79
2. Fundamentals of modern analytical techniques used in microbial biotechnology and related sciences /83
2.1. Confocal, fluorescent microscopy and spectrofluorimetry (S. Różalska) /85
2.2. Isotopic techniques (radioactive isotopes) (J. Długoński, S. Różalska) /93
2.3. Chromatography (R. Szewczyk) /96
2.4. Mass spectrometry (R. Szewczyk) /108
2.5. Atomic absorption spectroscopy (M. Słaba) /119
2.6. Modern digital techniques used to record changes in the environment (A. Długoński) /124
Literature /133
3. Determining the taxonomic affiliation of microorganisms /137
3.1. Genotypic techniques for differentiation and identification of bacteria (M. Krupiński) /139
3.2. Fungi of the phyla Mucoromycota, Ascomycota and Basidiomycota – morphological, biochemical features and genetic analysis (M. Słaba, S. Różalska) /156
3.3. Microbial biotyping by LC-MS/MS and MALDI-TOF/TOF methods (R. Szewczyk) /175
Literature /188
4. Industrial applications of microorganisms /191
4.1. Biosynthesis processes (J. Długoński) /193
4.2. Biotransformation processes (J. Długoński) /277
Literature /287
5. Microorganisms in environmental and human health protection /295
5.1. Revitalisation of degraded urban green areas (A. Długoński) /297
5.2. Microbiological analysis of polluted environments – Next generation sequencing (S. Różalska) /310
5.3. Biological wastewater treatment processes /317
5.4. Waste composting (A. Długoński, K. Lisowska) /325
5.5. Use of municipal green waste for energy production in local biogas and incineration plants and the synthesis of fungal laccases (A. Długoński, A. Góralczyk-Bińkowska) /330
5.6. Biodegradation of toxic xenobiotics (J. Długoński) /335
5.7. Microbiological elimination of heavy metals from the environment (M. Słaba, J. Nykiel-Szymańska) /362
5.8. Detoxification processes of polluted environments. Ecotoxicological tests (M. Krupiński) /371
5.9. Use of industrial waste in microbiological biotechnology (K. Paraszkiewicz, A. Góralczyk-Bińkowska, A. Jasińska) /377
5.10. Biodeterioration caused by fungi (S. Różalska, M. Słaba, A. Długoński) /383
5.11. Characteristics and use of ligninolytic enzymes produced by fungi in environmental protection, industry and medicine (A. Jasińska, A. Góralczyk-Bińkowska, A. Długoński) /391
5.12. Determination of antimicrobial properties of macromolecules (dendrimers) and newly synthesised silver compounds (A. Felczak, K. Zawadzka) /398
5.13. Entomopathogenic fungi and their use in biocontrol (S. Różalska) /403
5.14. Toxigenic fungi. Search for and identification of aflatoxins (K. Paraszkiewicz, M. Słaba, R. Szewczyk) /407
Literature /418
6. Omics in microbial biotechnology /431
6.1. Proteomics in microbiological analysis of xenobiotics degradation (R. Szewczyk) /433
6.2. Metabolomic analysis as a tool for multi-level characterisation of the biodegradation process (R. Szewczyk) /446
6.3. Application of lipidomics in the study of detoxification processes in microorganisms (P. Bernat) /452
6.4. Search for biomarkers in industry and medicine (R. Szewczyk) /459
6.5. Quantitative analysis of pesticides – multimethods (R. Szewczyk) /464
Literature /470
7. Media, buffers (K. Milczarek, N. Wrońska, A. Felczak) /473
7.1. Media /475
7.2. Buffers /494
Literature /496
8. Macroscopic and microscopic images of fungal strains applied at the Department of Industrial Microbiology and Biotechnology at the University of Łódź /497
8.1. Photographs of fungi cultured under laboratory conditions (K. Milczarek, S. Różalska) /499
8.2. Photographs of trees and wood infected by ligninolytic fungi (A. Długoński) /530
Literature /543
Authors /545

Microbialbiotechnologyinthelaboratoryandinpractce

C0Stabilisationofmicroorganismsbyimmobilisation

Materialsandmedia

1.Microorganism:Saccharomycescerevisiae.

2.Medium:wort12°Blgand8°Blg.

Reagents:sodiumalginate,carrageenan,0.2Msolution

ofCaCl

Aimoftheexercise

Comparisonoffermentatoncapacityoffreeyeastcellsandyeast

immobilisedingels.

Procedure

1.ImmobilisationofS.cerevisiaecellsinalginate.

Centrifugethe48-hshakenS.cerevisiaeculture

in12°Blgmaltwortfromthemedium,washwith

asolutionofsterilesalineandthencentrifuge

again.Thoroughlydisperseyeastprecipitatein

steriledistilledwater(in0.1originalvolume).In

theobtainedsuspension,counttheyeastcellsinthe

Thomachamberandgivetheirdensityper1mlofthe

immobilizate.Dividetheyeastprecipitateobtained

fromthecultureinto2equalparts;

b)preparea4%sterilesolutionofsodiumalginate;

c)mixtheyeastsuspensionwiththealginatesolutionat

avolumeratioof1:1;

putthecellsuspensioninthealginateinasterile

syringewithaneedle1mmindiameterandacutend;

dropthesuspensionintothesterile0.2MCaCl

.Leave

thebeadsinsolutionfor20min.Washthehardened

beadstwicewithsteriledistilledwater;

e)placethebeadsinaﬂaskcontaining100mlof8°Blgof

maltwort;transfertheﬂasktoathermostatat28°C;

carryoutanidenticalfermentationtestwithS.

cerevisiaefreecellsatthesameinitialdensity;

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Microbial biotechnology in the laboratory and practice

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