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Methodsofscreening,culturing,improvementandstorageofmicroorganisms...
Procedure
1.
ComparisonofgrowthrateofCunninghamellaechinulata
insubmergedandsurfacecultureinSabouraud
medium.
2.
SUBMERGEDCULTURE.7-daycultureofC.echinulata
onaslantedSabouraudmedium.Washof3mlofsterile
distilledwaterandtransfertheculturesuspensionto
20mlofsterileSabouraudliquidmediumplacedin
a100mlflask.Use6flasksseparatelyfortheexperiment.
Streakingiscarriedoutimmediatelyafterwashingthe
slants,cultureiscarriedoutonashakerat28°C,then
after0,6,9,12,18and24hofincubation:
a)
describethegrowthofmicroorganismsinthe
culture;
b)
filtertheculturefromeachflaskthroughthefilter
paperundervacuum,washwithsteriledistilledwater
anddryat105°Ctoconstantweight;
c)determinethemaximumspecificgrowthrate.
3.
SURFACECULTURE.7-daycultureofC.echinulataon
aslantedSabouraudmedium.Washof3mlofsterile
distilledwaterandtransfertheculturesuspensionto
20mlofsterileSabouraudmediumplacedina100ml
flask.Use6flasksseparatelyfortheexperiment.
Streakingiscarriedoutimmediatelyafterwashingthe
slants,thenafter12,24,36,48,60and72hofcultureit
isnecessaryto:
a)
describethegrowthofmicroorganismsinthe
culture;
b)
filtertheculturefromeachflaskthroughthefilter
paperundervacuum,washwithsteriledistilledwater
anddryat105°Ctoconstantweight;
c)determinethemaximumspecificgrowthrate.
Analysisofresults
Onthebasisoftheobtainedvaluesofthespecificgrowthrate,
explainthediferencesintherateofbiomassgrowthinbothtypes
ofculture.
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